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Molecular characterization of closteroviruses infecting Cordyline fruticosa L. in Hawaii.

ABSTRACT: In Hawaii, common green ti plants (Cordyline fruticosa L.) have been shown to harbor Cordyline virus 1 (CoV-1) which, along with Little cherry virus 1 (LChV-1), and Grapevine leafroll-associated virus 7 (GLRaV-7), form a distinct clade within the family Closteroviridae. Preliminary work has indicated that, aside from CoV-1, three additional closteroviruses may infect common green ti plants in Hawaii. In this study, pyrosequencing was used to characterize the genomes of closteroviruses infecting a single common green ti plant. The sequence data confirmed the presence of CoV-1 as well as three additional closteroviruses. Although all four viruses had the same general genome organization, the sequence divergence between the RNA-dependent RNA polymerase, heat shock protein 70 homolog, and coat protein ranged from 22 to 61%, indicating these represent four distinct closterovirus species. The names CoV-2, CoV-3, and CoV-4 are proposed for the three new viruses. Phylogenetic analyses placed CoV-2, CoV-3, and CoV-4 in the same clade as CoV-1, LChV-1, and GLRaV-7.

SUBMITTER: Melzer MJ

PROVIDER: S-EPMC3588190 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Molecular characterization of closteroviruses infecting Cordyline fruticosa L. in Hawaii.

Melzer Michael J MJ Sugano Jari S JS Uchida Janice Y JY Borth Wayne B WB Kawate Michael K MK Hu John S JS

Frontiers in microbiology 20130305

In Hawaii, common green ti plants (Cordyline fruticosa L.) have been shown to harbor Cordyline virus 1 (CoV-1) which, along with Little cherry virus 1 (LChV-1), and Grapevine leafroll-associated virus 7 (GLRaV-7), form a distinct clade within the family Closteroviridae. Preliminary work has indicated that, aside from CoV-1, three additional closteroviruses may infect common green ti plants in Hawaii. In this study, pyrosequencing was used to characterize the genomes of closteroviruses infecting ...[more]

PMID: 23467405

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Dataset Information

Molecular characterization of closteroviruses infecting Cordyline fruticosa L. in Hawaii.

Publications

Molecular characterization of closteroviruses infecting Cordyline fruticosa L. in Hawaii.

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