Ontology highlight
ABSTRACT:
SUBMITTER: Frese CK
PROVIDER: S-EPMC3588588 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Frese Christian K CK Zhou Houjiang H Taus Thomas T Altelaar A F Maarten AF Mechtler Karl K Heck Albert J R AJ Mohammed Shabaz S
Journal of proteome research 20130207 3
We recently introduced a novel scheme combining electron-transfer and higher-energy collision dissociation (termed EThcD), for improved peptide ion fragmentation and identification. We reasoned that phosphosite localization, one of the major hurdles in high-throughput phosphoproteomics, could also highly benefit from the generation of such EThcD spectra. Here, we systematically assessed the impact on phosphosite localization utilizing EThcD in comparison to methods employing either ETD or HCD, r ...[more]