Ontology highlight
ABSTRACT:
SUBMITTER: Yu M
PROVIDER: S-EPMC3589129 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Yu Miao M Hon Gary C GC Szulwach Keith E KE Song Chun-Xiao CX Zhang Liang L Kim Audrey A Li Xuekun X Dai Qing Q Shen Yin Y Park Beomseok B Min Jung-Hyun JH Jin Peng P Ren Bing B He Chuan C
Cell 20120517 6
The study of 5-hydroxylmethylcytosines (5hmC) has been hampered by the lack of a method to map it at single-base resolution on a genome-wide scale. Affinity purification-based methods cannot precisely locate 5hmC nor accurately determine its relative abundance at each modified site. We here present a genome-wide approach, Tet-assisted bisulfite sequencing (TAB-Seq), that when combined with traditional bisulfite sequencing can be used for mapping 5hmC at base resolution and quantifying the relati ...[more]