Phosphoglycerate mutases function as reverse regulated isoenzymes in Synechococcus elongatus PCC 7942.

ABSTRACT: Phosphoglycerate-mutase (PGM) is an ubiquitous glycolytic enzyme, which in eukaryotic cells can be found in different compartments. In prokaryotic cells, several PGMs are annotated/localized in one compartment. The identification and functional characterization of PGMs in prokaryotes is therefore important for better understanding of metabolic regulation. Here we introduce a method, based on a multi-level kinetic model of the primary carbon metabolism in cyanobacterium Synechococcus elongatus PCC 7942, that allows the identification of a specific function for a particular PGM. The strategy employs multiple parameter estimation runs in high CO2, combined with simulations testing a broad range of kinetic parameters against the changes in transcript levels of annotated PGMs. Simulations are e

SUBMITTER: Jablonsky J 

PROVIDER: S-EPMC3590821 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature