Unknown

Dataset Information

0

Molecular characterization of high-level-cholera-toxin-producing El Tor variant Vibrio cholerae strains in the Zanzibar Archipelago of Tanzania.


ABSTRACT: Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-field gel electrophoresis (PFGE) analysis.

SUBMITTER: Naha A 

PROVIDER: S-EPMC3592071 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

Molecular characterization of high-level-cholera-toxin-producing El Tor variant Vibrio cholerae strains in the Zanzibar Archipelago of Tanzania.

Naha A A   Chowdhury G G   Ghosh-Banerjee J J   Senoh M M   Takahashi T T   Ley B B   Thriemer K K   Deen J J   Seidlein L V LV   Ali S M SM   Khatib A A   Ramamurthy T T   Nandy R K RK   Nair G B GB   Takeda Y Y   Mukhopadhyay A K AK  

Journal of clinical microbiology 20130116 3


Analysis of 1,180 diarrheal stool samples in Zanzibar detected 247 Vibrio cholerae O1, Ogawa strains in 2009. Phenotypic traits and PCR-based detection of rstR, rtxC, and tcpA alleles showed that they belonged to the El Tor biotype. Genetic analysis of ctxB of these strains revealed that they were classical type, and production of classical cholera toxin B (CTB) was confirmed by Western blotting. These strains produced more CT than the prototype El Tor and formed a separate cluster by pulsed-fie  ...[more]

Similar Datasets

| S-EPMC7214932 | biostudies-literature
| S-EPMC2681878 | biostudies-literature
| S-EPMC1698305 | biostudies-literature
| S-EPMC1594734 | biostudies-literature
| S-EPMC5088018 | biostudies-literature
2016-03-23 | GSE72473 | GEO
2016-03-23 | GSE72472 | GEO
2016-03-23 | GSE72471 | GEO
2012-04-13 | GSE36373 | GEO
| S-EPMC4453562 | biostudies-literature