Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing.
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ABSTRACT:
SUBMITTER: VanInsberghe D
PROVIDER: S-EPMC3592214 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Publications
Isolation of a substantial proportion of forest soil bacterial communities detected via pyrotag sequencing.
VanInsberghe David D Hartmann Martin M Stewart Gordon R GR Mohn William W WW
Applied and environmental microbiology 20130111 6
We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units. ...[more]
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