Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.
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ABSTRACT:
SUBMITTER: Tolun AA
PROVIDER: S-EPMC3595192 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
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Allantoin in human urine quantified by ultra-performance liquid chromatography-tandem mass spectrometry.
Tolun Adviye A AA Zhang Haoyue H Il'yasova Dora D Sztáray Judit J Young Sarah P SP Millington David S DS
Analytical biochemistry 20100331 2
Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography-tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and ...[more]
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