Unknown

Dataset Information

0

TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats.


ABSTRACT: The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-1N) resembling human mesangioproliferative glomerulonephritis have been explored for many years; however, the molecular mechanisms of GMC proliferation and ECM production remain unclear. Our previous studies have demonstrated that the thrombospondin-1 (TSP-1) gene was involved in mediating rat GMC proliferation and ECM synthesis induced by sublytic C5b-9 in vitro. In the present study, the roles of the TSP-1 gene in GMC proliferation, ECM production, and urinary protein secretion in Thy-1N rats were determined by using TSP-1 small hairpin RNA, and the results revealed that silencing of the TSP-1 gene in rat renal tissues could diminish GMC proliferation (P < 0.01) and ECM secretion (P < 0.01) as well as urinary protein secretion (P < 0.05) in Thy-1N rats. Together, the current findings suggested that TSP-1 gene expression was required for GMC proliferation and ECM production in Thy-1N rats.

SUBMITTER: Qiu W 

PROVIDER: S-EPMC3596719 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats.

Qiu Wen W   Li Yan Y   Zhou Jianbo J   Zhao Chenhui C   Zhang Jing J   Shan Kai K   Zhao Dan D   Wang Yingwei Y  

Journal of biomedical research 20111101 6


The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-1N) resembling human mesangioproliferative glomerulonephritis have been explored for many years; however, the molecular mechanisms of GMC proliferation and ECM production remain unclear. Our previous studies have demonstrated that the thrombospondin-1 (TSP-1) gene was involved in mediating rat GMC proliferation and ECM synthesis induced by sublytic C5b-9 in vitro  ...[more]

Similar Datasets

| S-EPMC8168418 | biostudies-literature
| S-EPMC5583188 | biostudies-literature
| S-EPMC8102557 | biostudies-literature
| S-EPMC3351322 | biostudies-literature
| S-EPMC6528942 | biostudies-literature
| S-EPMC10817934 | biostudies-literature
2006-04-01 | GSE3221 | GEO
| S-EPMC7533486 | biostudies-literature
| S-EPMC4044709 | biostudies-literature
| S-EPMC5825286 | biostudies-literature