Ontology highlight
ABSTRACT:
SUBMITTER: Evrin C
PROVIDER: S-EPMC3597701 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Evrin Cecile C Fernández-Cid Alejandra A Zech Juergen J Herrera M Carmen MC Riera Alberto A Clarke Pippa P Brill Shlomo S Lurz Rudi R Speck Christian C
Nucleic acids research 20130201 5
The origin recognition complex (ORC) of Saccharomyces cerevisiae binds origin DNA and cooperates with Cdc6 and Cdt1 to load the replicative helicase MCM2-7 onto DNA. Helicase loading involves two MCM2-7 hexamers that assemble into a double hexamer around double-stranded DNA. This reaction requires ORC and Cdc6 ATPase activity, but it is unknown how these proteins control MCM2-7 double hexamer formation. We demonstrate that mutations in Cdc6 sensor-2 and Walker A motifs, which are predicted to af ...[more]