A consensus mechanism for Radical SAM-dependent dehydrogenation? BtrN contains two [4Fe-4S] clusters.
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SUBMITTER: Grove TL
PROVIDER: S-EPMC3598582 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
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A consensus mechanism for Radical SAM-dependent dehydrogenation? BtrN contains two [4Fe-4S] clusters.
Grove Tyler L TL Ahlum Jessica H JH Sharma Priya P Krebs Carsten C Booker Squire J SJ
Biochemistry 20100501 18
BtrN catalyzes the two-electron oxidation of the C3 secondary alcohol of 2-deoxy-scyllo-inosamine to the corresponding ketone and is a member of a subclass of radical S-adenosylmethionine (SAM) enzymes called radical SAM (RS) dehydrogenases. Like all RS enzymes, BtrN contains a [4Fe-4S] cluster that delivers an electron to SAM, inducing its cleavage to the common intermediate in RS reactions, the 5'-deoxyadenosyl 5'-radical. In this work, we show that BtrN contains an additional [4Fe-4S] cluster ...[more]
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