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Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase.


ABSTRACT: Generation of meiotic crossovers in many eukaryotes requires the elimination of anti-crossover activities by using the Msh4-Msh5 heterodimer to block helicases. Msh4 and Msh5 have been lost from the flies Drosophila and Glossina, but we identified a complex of minichromosome maintenance (MCM) proteins that functionally replace Msh4-Msh5. We found that REC, an ortholog of MCM8 that evolved under strong positive selection in flies, interacts with MEI-217 and MEI-218, which arose from a previously undescribed metazoan-specific MCM protein. Meiotic crossovers were reduced in Drosophila rec, mei-217, and mei-218 mutants; however, removal of the Bloom syndrome helicase (BLM) ortholog restored crossovers. Thus, MCMs were co-opted into a novel complex that replaced the meiotic pro-crossover function of Msh4-Msh5 in flies.

SUBMITTER: Kohl KP 

PROVIDER: S-EPMC3599781 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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Evolution of an MCM complex in flies that promotes meiotic crossovers by blocking BLM helicase.

Kohl Kathryn P KP   Jones Corbin D CD   Sekelsky Jeff J  

Science (New York, N.Y.) 20121201 6112


Generation of meiotic crossovers in many eukaryotes requires the elimination of anti-crossover activities by using the Msh4-Msh5 heterodimer to block helicases. Msh4 and Msh5 have been lost from the flies Drosophila and Glossina, but we identified a complex of minichromosome maintenance (MCM) proteins that functionally replace Msh4-Msh5. We found that REC, an ortholog of MCM8 that evolved under strong positive selection in flies, interacts with MEI-217 and MEI-218, which arose from a previously  ...[more]

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2022-08-24 | GSE201366 | GEO