Ontology highlight
ABSTRACT:
SUBMITTER: Gahlmann A
PROVIDER: S-EPMC3599789 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Gahlmann Andreas A Ptacin Jerod L JL Grover Ginni G Quirin Sean S von Diezmann Lexy L Lee Marissa K MK Backlund Mikael P MP Shapiro Lucy L Piestun Rafael R Moerner W E WE
Nano letters 20130225 3
We demonstrate quantitative multicolor three-dimensional (3D) subdiffraction imaging of the structural arrangement of fluorescent protein fusions in living Caulobacter crescentus bacteria. Given single-molecule localization precisions of 20-40 nm, a flexible locally weighted image registration algorithm is critical to accurately combine the super-resolution data with <10 nm error. Surface-relief dielectric phase masks implement a double-helix response at two wavelengths to distinguish two differ ...[more]