Family-specific degenerate primer design: a tool to design consensus degenerated oligonucleotides.
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ABSTRACT:
SUBMITTER: Iserte JA
PROVIDER: S-EPMC3600133 | biostudies-literature | 2013
REPOSITORIES: biostudies-literature
Publications
Family-specific degenerate primer design: a tool to design consensus degenerated oligonucleotides.
Iserte Javier Alonso JA Stephan Betina Ines BI Goñi Sandra Elizabeth SE Borio Cristina Silvia CS Ghiringhelli Pablo Daniel PD Lozano Mario Enrique ME
Biotechnology research international 20130221
Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer software, for which the starting point is a multiple alignment of related amino acids or nucleotide sequences. To assess their efficiency, four different genome collections were used, covering a wide range of genomic lengths: Arenavirus (10 × 10(4) ...[more]
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