Ontology highlight
ABSTRACT:
SUBMITTER: Zong C
PROVIDER: S-EPMC3600412 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Science (New York, N.Y.) 20121201 6114
Kindred cells can have different genomes because of dynamic changes in DNA. Single-cell sequencing is needed to characterize these genomic differences but has been hindered by whole-genome amplification bias, resulting in low genome coverage. Here, we report on a new amplification method-multiple annealing and looping-based amplification cycles (MALBAC)-that offers high uniformity across the genome. Sequencing MALBAC-amplified DNA achieves 93% genome coverage ≥1x for a single human cell at 25x m ...[more]