Ontology highlight
ABSTRACT:
SUBMITTER: Siegrist MS
PROVIDER: S-EPMC3601600 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Siegrist M Sloan MS Whiteside Sarah S Jewett John C JC Aditham Arjun A Cava Felipe F Bertozzi Carolyn R CR
ACS chemical biology 20130111 3
Peptidoglycan (PG) is an essential component of the bacterial cell wall. Although experiments with organisms in vitro have yielded a wealth of information on PG synthesis and maturation, it is unclear how these studies translate to bacteria replicating within host cells. We report a chemical approach for probing PG in vivo via metabolic labeling and bioorthogonal chemistry. A wide variety of bacterial species incorporated azide and alkyne-functionalized d-alanine into their cell walls, which we ...[more]