Ontology highlight
ABSTRACT:
SUBMITTER: Musselman CA
PROVIDER: S-EPMC3603146 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Musselman Catherine A CA Avvakumov Nikita N Watanabe Reiko R Abraham Christopher G CG Lalonde Marie-Eve ME Hong Zehui Z Allen Christopher C Roy Siddhartha S Nuñez James K JK Nickoloff Jac J Kulesza Caroline A CA Yasui Akira A Côté Jacques J Kutateladze Tatiana G TG
Nature structural & molecular biology 20121111 12
The PHD finger protein 1 (PHF1) is essential in epigenetic regulation and genome maintenance. Here we show that the Tudor domain of human PHF1 binds to histone H3 trimethylated at Lys36 (H3K36me3). We report a 1.9-Å resolution crystal structure of the Tudor domain in complex with H3K36me3 and describe the molecular mechanism of H3K36me3 recognition using NMR. Binding of PHF1 to H3K36me3 inhibits the ability of the Polycomb PRC2 complex to methylate Lys27 of histone H3 in vitro and in vivo. Laser ...[more]