Ontology highlight
ABSTRACT:
SUBMITTER: Zhao L
PROVIDER: S-EPMC3604897 | biostudies-literature | 2012 Jan
REPOSITORIES: biostudies-literature
Zhao Liangjun L Dewage Sajeewa W SW Bell Michael J MJ Chang Keng-Ming KM Fatma Shirin S Joshi Nilesh N Silva Gayathri G Cisneros G Andrés GA Hendrickson Tamara L TL
Biochemistry 20111229 1
The Helicobacter pylori (Hp) Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase (AdT) plays important roles in indirect aminoacylation and translational fidelity. AdT has two active sites, in two separate subunits. Kinetic studies have suggested that interdomain communication occurs between these subunits; however, this mechanism is not well understood. To explore domain-domain communication in AdT, we adapted an assay and optimized it to kinetically characterize the kinase activity of Hp AdT. This as ...[more]