Detection of orexin A neuropeptide in biological fluids using a zinc oxide field effect transistor.

ABSTRACT: Biomarkers which are indicative of acute physiological and emotional states are studied in a number of different areas in cognitive neuroscience. Currently, many cognitive studies are conducted based on programmed tasks followed by timed biofluid sampling, central laboratory processing, and followed by data analysis. In this work, we present a sensor platform capable of rapid biomarker detection specific for detecting neuropeptide orexin A, found in blood and saliva and known as an indicator of fatigue and cognitive performance. A peptide recognition element that selectively binds to orexin A was designed, characterized, and functionalized onto a zinc oxide field effect transistor to enable rapid detection. The detection limit using the sensor platform was sub-picomolar in water, and picom

SUBMITTER: Hagen J 

PROVIDER: S-EPMC3605810 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature