Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line.

ABSTRACT:

Background

Silver nanoparticles (AgNP), the most popular nano-compounds, possess unique properties. Albizia adianthifolia (AA) is a plant of the Fabaceae family that is rich in saponins. The biological properties of a novel AgNP, synthesized from an aqueous leaf extract of AA (AA(AgNP)), were investigated on A549 lung cells. Cell viability was determined by the MTT assay. Cellular oxidative status (lipid peroxidation and glutathione (GSH) levels), ATP concentration, caspase-3/-7, -8 and -9 activities were determined. Apoptosis, mitochondrial (mt) membrane depolarization (flow cytometry) and DNA fragmentation (comet assay) were assessed. The expression of CD95 receptors, p53, bax, PARP-1 and smac/DIABLO was evaluated by flow cytometry and/or western blotting.

Results

Silver nanoparticles of AA caused a dose-dependent decrease in cell viability with a significant increase in lipid peroxidation (5-fold vs. control; p?=?0.0098) and decreased intracellular GSH (p?=?0.1184). A significant 2.5-fold decrease in cellular ATP was observed upon AA(AgNP) exposure (p?=?0.0040) with a highly significant elevation in mt depolarization (3.3-fold vs. control; p?ConclusionData suggests that AA(AgNP) induces cell death in the A549 lung cells via the mt mediated intrinsic apoptotic program. Further investigation is required to potentiate the use of this novel compound in cancer therapy trials.

SUBMITTER: Govender R 

PROVIDER: S-EPMC3606379 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature