RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane.
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ABSTRACT:
SUBMITTER: O'Neill BP
PROVIDER: S-EPMC3606522 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Publications
RNAi-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane.
O'Neill Brian P BP Purnell Matthew P MP Nielsen Lars K LK Brumbley Stevens M SM
SpringerPlus 20121221 1
To engineer trehalose metabolism in sugarcane (Saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (TPSP), to increase trehalose biosynthesis and an RNAi transgene specific for trehalase, to abrogate trehalose catabolism. In RNAi-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. In TPSP lines trehalase activity was significantly higher. No events of co-integratio ...[more]
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