Crystallization and preliminary X-ray analysis of human leukocyte cell-derived chemotaxin 2 (LECT2).

ABSTRACT: Human leukocyte cell-derived chemotaxin 2 (LECT2) is a chemotactic factor for neutrophils that plays multifunctional roles in liver regeneration, regulation of neuritic development and proliferation of chondrocytes and osteoblasts. In addition, the C-terminal region of LECT2 belongs to the zinc metalloendopeptidase M23 (PF01551) family. Purified LECT2 was crystallized using the sitting-drop vapour-diffusion method at 293?K. Crystals of selenomethionine-substituted LECT2 that diffracted X-rays to 1.94?Å resolution were obtained using a reservoir solution consisting of 0.2?M ammonium sulfate, 0.1?M HEPES pH 7.5, 25%(w/v) PEG 8000. The crystal belonged to space group P2?2?2?, with unit-cell parameters a=59.4, b=63.5, c=64.0?Å. The calculated Matthews coefficient (VM=2.10?Å3?Da(-1), solvent content 40%) indicates that the crystal consists of two molecules per asymmetric unit.

SUBMITTER: Zheng H 

PROVIDER: S-EPMC3606582 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature