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Genetic analysis of polymerase complex (PA, PB1 and PB2) genes of H9N2 avian influenza viruses from Iran (1999 to 2009).


ABSTRACT: OBJECTIVE: To determine the molecular characterization of Polymerase complex (PA, PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relationship of Iranian H9N2 viruses and other Asian viruses. METHODS: The Polymerase complex (PA, PB1 and PB2) genes from seven isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified (by RT-PCR method) and sequenced. Nucleotide sequences (Open Reading Frame: orf) of the PA, PB1 and PB2 genes were used for phylogenetic tree construction. RESULTS: Most PB2 and PA genes of the H9N2 viruses isolated in 2008-2009 belonged to the unknown avian sublineage which grouped with the 2004 Pakistani H7N3 viruses. The PB1 genes of Iranian viruses indicated greater genetic diversity and shared a high level of similarity to PB1 genes from either H5 or H7 subtypes with compared to established H9N2 Eurasian sublineages. CONCLUSIONS: Our findings demonstrated that the H9N2 viruses in Iran exhibit striking reassortment which has led to the generation of new genotypes.

SUBMITTER: Soltanialvar M 

PROVIDER: S-EPMC3609240 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Genetic analysis of polymerase complex (PA, PB1 and PB2) genes of H9N2 avian influenza viruses from Iran (1999 to 2009).

Soltanialvar Masoud M   Goodarzi Reza R   Goodarzi Reza R   Akbarnejad Farshad F  

Asian Pacific journal of tropical biomedicine 20121101 11


<h4>Objective</h4>To determine the molecular characterization of Polymerase complex (PA, PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relationship of Iranian H9N2 viruses and other Asian viruses.<h4>Methods</h4>The Polymerase complex (PA, PB1 and PB2) genes from seven isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified (by RT-PCR method) and sequenced. Nucleotide sequences (Open Reading Frame: orf) of the PA, PB1 and PB2 genes  ...[more]

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