Genetic analysis of polymerase complex (PA, PB1 and PB2) genes of H9N2 avian influenza viruses from Iran (1999 to 2009).

ABSTRACT:

Objective

To determine the molecular characterization of Polymerase complex (PA, PB1 and PB2) genes of H9N2 avian influenza viruses and the genetic relationship of Iranian H9N2 viruses and other Asian viruses.

Methods

The Polymerase complex (PA, PB1 and PB2) genes from seven isolates of H9N2 viruses isolated from commercial chickens in Iran during 2008-2009 were amplified (by RT-PCR method) and sequenced. Nucleotide sequences (Open Reading Frame: orf) of the PA, PB1 and PB2 genes were used for phylogenetic tree construction.

Results

Most PB2 and PA genes of the H9N2 viruses isolated in 2008-2009 belonged to the unknown avian sublineage which grouped with the 2004 Pakistani H7N3 viruses. The PB1 genes of Iranian viruses indicated greater genetic diversity and shared a

SUBMITTER: Soltanialvar M 

PROVIDER: S-EPMC3609240 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature