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An Ecofriendly synthesis of silver nano-bioconjugates by Penicillium citrinum (MTCC9999) and its antimicrobial effect.

ABSTRACT: This report provides for the first time a novel environment friendly extracellular synthesis of stable silver nano-bioconjugates (SNBCs) at room temperature at pH 5.0 using Penicillium citrinum MTCC 9999 biomass. The UV-Visible spectral scan of dispersed SNBCs solution showed absorption in the region 340-450 nm due to surface plasma resonance (SPR). Typical Transmission Electron Microscopic (TEM) images showed that although two populations were present but most of them were in 20-30 nm range. Average zeta potential of SNBCs was -21 mV suggesting some biomolecules capped the nanoparticles imparting a net negative charge over it. FTIR analysis also showed that biomolecules were involved in stabilization. SNBCs showed strong antibacterial activity against both Gram positive (Bacillus subtilis) and Gram negative bacteria (Escherichia coli). SNBCs also showed strong antifungal activity as assessed against Schizosaccharomyces pombe. In the case of E. coli the minimum inhibitory concentrations (MIC) of SNBCs was 4 ?g/ml while in B. subtilis it was 8 ?g/ml. In the case of E. coli the minimum bactericidal concentrations (MBC) of SNBCs was 8 ?g/ml while in B. subtilis it was 32 ?g/ml. The SNBCs exerted its antibacterial and antifungal activity through generation of reactive oxygen species (ROS) inside the cell.

SUBMITTER: Goswami AM

PROVIDER: S-EPMC3610205 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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An Ecofriendly synthesis of silver nano-bioconjugates by Penicillium citrinum (MTCC9999) and its antimicrobial effect.

Goswami Achintya Mohan AM Sarkar Tuhin Subhra TS Ghosh Sanjay S

AMB Express 20130223 1

This report provides for the first time a novel environment friendly extracellular synthesis of stable silver nano-bioconjugates (SNBCs) at room temperature at pH 5.0 using Penicillium citrinum MTCC 9999 biomass. The UV-Visible spectral scan of dispersed SNBCs solution showed absorption in the region 340-450 nm due to surface plasma resonance (SPR). Typical Transmission Electron Microscopic (TEM) images showed that although two populations were present but most of them were in 20-30 nm range. Av ...[more]

PMID: 23433075

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Project description:BackgroundEndophytic fungi are known plant symbionts. They produce a variety of beneficial metabolites for plant growth and survival, as well as defend their hosts from attack of certain pathogens. Coastal dunes are nutrient deficient and offer harsh, saline environment for the existing flora and fauna. Endophytic fungi may play an important role in plant survival by enhancing nutrient uptake and producing growth-promoting metabolites such as gibberellins and auxins. We screened roots of Ixeris repenes (L.) A. Gray, a common dune plant, for the isolation of gibberellin secreting endophytic fungi.ResultsWe isolated 15 endophytic fungi from the roots of Ixeris repenes and screened them for growth promoting secondary metabolites. The fungal isolate IR-3-3 gave maximum plant growth when applied to waito-c rice and Atriplex gemelinii seedlings. Analysis of the culture filtrate of IR-3-3 showed the presence of physiologically active gibberellins, GA1, GA3, GA4 and GA7 (1.95 ng/ml, 3.83 ng/ml, 6.03 ng/ml and 2.35 ng/ml, respectively) along with other physiologically inactive GA5, GA9, GA12, GA15, GA19, GA20 and, GA24. The plant growth promotion and gibberellin producing capacity of IR-3-3 was much higher than the wild type Gibberella fujikuroi, which was taken as control during present study. GA5, a precursor of bioactive GA3 was reported for the first time in fungi. The fungal isolate IR-3-3 was identified as a new strain of Penicillium citrinum (named as P. citrinum KACC43900) through phylogenetic analysis of 18S rDNA sequence.ConclusionIsolation of new strain of Penicillium citrinum from the sand dune flora is interesting as information on the presence of Pencillium species in coastal sand dunes is limited. The plant growth promoting ability of this fungal strain may help in conservation and revegetation of the rapidly eroding sand dune flora. Penicillium citrinum is already known for producing mycotoxin citrinin and cellulose digesting enzymes like cellulase and endoglucanase, as well as xylulase. Gibberellins producing ability of this fungus and the discovery about the presence of GA5 will open new aspects of research and investigations.

Dataset Information

An Ecofriendly synthesis of silver nano-bioconjugates by Penicillium citrinum (MTCC9999) and its antimicrobial effect.

Publications

An Ecofriendly synthesis of silver nano-bioconjugates by Penicillium citrinum (MTCC9999) and its antimicrobial effect.

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