MiR-145 and miR-143 regulate odontoblast differentiation through targeting Klf4 and Osx genes in a feedback loop.

ABSTRACT: Dentin tissue is derived from mesenchymal cells induced into the odontoblast lineage. The differentiation of odontoblasts is a complex process regulated by several transcriptional factor signaling transduction pathways. However, post-translational regulation of these factors during dentinogenesis remains unclear. To further explore the mechanisms, we investigated the role of microRNA (miRNA) during odontoblast differentiation. We profiled the miRNA expression pattern during mouse odontoblast differentiation using a microarray assay and identified that miR-145 and miR-143 were down-regulated during this process. In situ hybridization verified that the two miRNAs were gradually decreased during mouse odontoblast differentiation. Loss-of-function and gain-of-function experiments revealed that

SUBMITTER: Liu H 

PROVIDER: S-EPMC3610997 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature