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State-dependent FRET reports calcium- and voltage-dependent gating-ring motions in BK channels.


ABSTRACT: Large-conductance voltage- and calcium-dependent potassium channels (BK, "Big K+") are important controllers of cell excitability. In the BK channel, a large C-terminal intracellular region containing a "gating-ring" structure has been proposed to transduce Ca(2+) binding into channel opening. Using patch-clamp fluorometry, we have investigated the calcium and voltage dependence of conformational changes of the gating-ring region of BK channels, while simultaneously monitoring channel conductance. Fluorescence resonance energy transfer (FRET) between fluorescent protein inserts indicates that Ca(2+) binding produces structural changes of the gating ring that are much larger than those predicted by current X-ray crystal structures of isolated gating rings.

SUBMITTER: Miranda P 

PROVIDER: S-EPMC3612663 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature

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State-dependent FRET reports calcium- and voltage-dependent gating-ring motions in BK channels.

Miranda Pablo P   Contreras Jorge E JE   Plested Andrew J R AJ   Sigworth Fred J FJ   Holmgren Miguel M   Giraldez Teresa T  

Proceedings of the National Academy of Sciences of the United States of America 20130311 13


Large-conductance voltage- and calcium-dependent potassium channels (BK, "Big K+") are important controllers of cell excitability. In the BK channel, a large C-terminal intracellular region containing a "gating-ring" structure has been proposed to transduce Ca(2+) binding into channel opening. Using patch-clamp fluorometry, we have investigated the calcium and voltage dependence of conformational changes of the gating-ring region of BK channels, while simultaneously monitoring channel conductanc  ...[more]

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