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Structure of the hypothetical DUF1811-family protein GK0453 from Geobacillus kaustophilus HTA426.

ABSTRACT: The crystal structure of a conserved hypothetical protein, GK0453, from Geobacillus kaustophilus has been determined to 2.2 Å resolution. The crystal belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 75.69, c = 64.18 Å. The structure was determined by the molecular-replacement method and was refined to a final R factor of 22.6% (R(free) = 26.3%). Based on structural homology, the GK0453 protein possesses two independent binding sites and hence it may simultaneously interact with two proteins or with a protein and a nucleic acid.

SUBMITTER: Padmanabhan B

PROVIDER: S-EPMC3614154 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Structure of the hypothetical DUF1811-family protein GK0453 from Geobacillus kaustophilus HTA426.

Padmanabhan Balasundaram B Nakamura Yoshihiro Y Antonyuk Svetlana V SV Strange Richard W RW Hasnain S Samar SS Yokoyama Shigeyuki S Bessho Yoshitaka Y

Acta crystallographica. Section F, Structural biology and crystallization communications 20130328 Pt 4

The crystal structure of a conserved hypothetical protein, GK0453, from Geobacillus kaustophilus has been determined to 2.2 Å resolution. The crystal belonged to space group P4(3)2(1)2, with unit-cell parameters a = b = 75.69, c = 64.18 Å. The structure was determined by the molecular-replacement method and was refined to a final R factor of 22.6% (R(free) = 26.3%). Based on structural homology, the GK0453 protein possesses two independent binding sites and hence it may simultaneously interact w ...[more]

PMID: 23545635

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