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Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex.

ABSTRACT: The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 Å.

SUBMITTER: Naumann PT

PROVIDER: S-EPMC3614169 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Purification, crystallization and preliminary crystallographic analysis of a 4-thiouridine synthetase-RNA complex.

Naumann Peter Thomas PT Lauhon Charles T CT Ficner Ralf R

Acta crystallographica. Section F, Structural biology and crystallization communications 20130328 Pt 4

The sulfurtransferase 4-thiouridine synthetase (ThiI) is involved in the ATP-dependent modification of U8 in tRNA. ThiI from Thermotoga maritima was cloned, overexpressed and purified. A complex comprising ThiI and a truncated tRNA was prepared and crystallized, and X-ray diffraction data were collected to a resolution of 3.5 Å. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 102.9, b = 112.8, c = 132.8 Å. ...[more]

PMID: 23545650

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