Ontology highlight
ABSTRACT:
SUBMITTER: Handa S
PROVIDER: S-EPMC3614408 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Handa Sumit S Spradling Tyler J TJ Dempsey Daniel R DR Merkler David J DJ
Protein expression and purification 20120425 1
Most mammalian bioactive peptides possess a C-terminal amino acid amide moiety. The presence of the C-terminal amide is a significant impediment to the recombinant production of α-amidated peptides. α-Amidated peptides are produced in vivo by the enzymatic cleavage of a precursor with a C-terminal glycine residue. Peptidylglycine α-hydroxylating monooxygenase catalyzes the key step in the oxidation of the glycine-extended precursors to the α-amidated peptide. Herein, we detail the production of ...[more]