Ontology highlight
ABSTRACT:
SUBMITTER: Gao L
PROVIDER: S-EPMC3615549 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Gao Liang L Shao Lin L Higgins Christopher D CD Poulton John S JS Peifer Mark M Davidson Michael W MW Wu Xufeng X Goldstein Bob B Betzig Eric E
Cell 20121201 6
Optical imaging of the dynamics of living specimens involves tradeoffs between spatial resolution, temporal resolution, and phototoxicity, made more difficult in three dimensions. Here, however, we report that rapid three-dimensional (3D) dynamics can be studied beyond the diffraction limit in thick or densely fluorescent living specimens over many time points by combining ultrathin planar illumination produced by scanned Bessel beams with super-resolution structured illumination microscopy. We ...[more]