Multiplex protein detection with DNA readout via mass spectrometry.
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ABSTRACT:
SUBMITTER: Flanigon J
PROVIDER: S-EPMC3616745 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
Publications
Multiplex protein detection with DNA readout via mass spectrometry.
Flanigon James J Kamali-Moghaddam Masood M Burbulis Ian I Annink Carla C Steffen Martin M Oeth Paul P Brent Roger R van den Boom Dirk D Landegren Ulf U Cantor Charles C
New biotechnology 20121128 2
Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wi ...[more]
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