Ontology highlight
ABSTRACT:
SUBMITTER: Flanigon J
PROVIDER: S-EPMC3616745 | biostudies-literature | 2013 Jan
REPOSITORIES: biostudies-literature
New biotechnology 20121128 2
Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wi ...[more]