Dataset Information

Mycobacterium tuberculosis responds to chloride and pH as synergistic cues to the immune status of its host cell.

ABSTRACT: The ability of Mycobacterium tuberculosis (Mtb) to thrive in its phagosomal niche is critical for its establishment of a chronic infection. This requires that Mtb senses and responds to intraphagosomal signals such as pH. We hypothesized that Mtb would respond to additional intraphagosomal factors that correlate with maturation. Here, we demonstrate that [Cl?] and pH correlate inversely with phagosome maturation, and identify Cl? as a novel environmental cue for Mtb. Mtb responds to Cl? and pH synergistically, in part through the activity of the two-component regulator phoPR. Following identification of promoters responsive to Cl? and pH, we generated a reporter Mtb strain that detected immune-mediated changes in the phagosomal environment during infection in a mouse model. Our study establishes Cl? and pH as linked environmental cues for Mtb, and illustrates the utility of reporter bacterial strains for the study of Mtb-host interactions in vivo.

SUBMITTER: Tan S

PROVIDER: S-EPMC3616970 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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Mycobacterium tuberculosis responds to chloride and pH as synergistic cues to the immune status of its host cell.

Tan Shumin S Sukumar Neelima N Abramovitch Robert B RB Parish Tanya T Russell David G DG

PLoS pathogens 20130404 4

The ability of Mycobacterium tuberculosis (Mtb) to thrive in its phagosomal niche is critical for its establishment of a chronic infection. This requires that Mtb senses and responds to intraphagosomal signals such as pH. We hypothesized that Mtb would respond to additional intraphagosomal factors that correlate with maturation. Here, we demonstrate that [Cl⁻] and pH correlate inversely with phagosome maturation, and identify Cl⁻ as a novel environmental cue for Mtb. Mtb responds to Cl⁻ and pH s ...[more]

PMID: 23592993

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Project description:Organisms from temperate ecosystems experience a cyclic alternation of favorable seasons, when they can grow and develop, and unfavorable periods, characterized by low temperatures and reduced resource availability. A common adaptation to these changing conditions is to undergo a state of metabolic arrest triggered by environmental cues (e.g. diapause) during the unfavorable periods. Altered environmental conditions resulting from global change can expose organisms to contradictory cues, potentially triggering maladaptive responses. Here, I compared the performance of an oligophagous butterfly when experiencing consistent vs contradictory environmental cues by manipulating temperature, daylength, and host plant in the laboratory. I implemented a fully factorial design with realistic temperature and photoperiodic regimes to resemble environmental conditions during mid-summer and the summer-autumn transition within the focal species' range. To assess the role of host plant at mediating the effects of abiotic factors, larvae were fed foliage of either a high or a low-quality host species. Decreasing daylength was the primary cue inducing diapause; however, feeding on a low-quality host at low temperatures also induced diapause in larvae growing under constant summer daylength. Conversely, exposure to high temperatures while feeding on a high-quality host occasionally overruled the diapause-inducing effect of decreasing daylength. Feeding on a high-quality host mitigated the lethal effects of cold, but not of hot temperatures. In addition, exposure to cold temperatures resulted in a significant reduction of pupal mass only under decreasing daylength. These results indicate that responses to environmental stressors in this multivoltine butterfly differ across the growing season according to the eco-physiological state of individuals (whether they undergo direct development or diapause). Traits related to oligophagy, such as sensitivity to multiple cues for diapause induction, as well as some of its consequences, such as the occurrence of overlapping generations, are likely to mitigate some of the detrimental effects of global change.

Dataset Information

Mycobacterium tuberculosis responds to chloride and pH as synergistic cues to the immune status of its host cell.

Publications

Mycobacterium tuberculosis responds to chloride and pH as synergistic cues to the immune status of its host cell.

Similar Datasets

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