Ontology highlight
ABSTRACT:
SUBMITTER: Nambu S
PROVIDER: S-EPMC3617252 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Nambu Shusuke S Matsui Toshitaka T Goulding Celia W CW Takahashi Satoshi S Ikeda-Saito Masao M
The Journal of biological chemistry 20130218 14
MhuD is an oxygen-dependent heme-degrading enzyme from Mycobacterium tuberculosis with high sequence similarity (∼45%) to Staphylococcus aureus IsdG and IsdI. Spectroscopic and mutagenesis studies indicate that the catalytically active 1:1 heme-MhuD complex has an active site structure similar to those of IsdG and IsdI, including the nonplanarity (ruffling) of the heme group bound to the enzyme. Distinct from the canonical heme degradation, we have found that the MhuD catalysis does not generate ...[more]