Ontology highlight
ABSTRACT:
SUBMITTER: Afonin KA
PROVIDER: S-EPMC3618561 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Afonin Kirill A KA Viard Mathias M Martins Angelica N AN Lockett Stephen J SJ Maciag Anna E AE Freed Eric O EO Heldman Eliahu E Jaeger Luc L Blumenthal Robert R Shapiro Bruce A BA
Nature nanotechnology 20130331 4
Split-protein systems, an approach that relies on fragmentation of proteins with their further conditional re-association to form functional complexes, are increasingly used for various biomedical applications. This approach offers tight control of protein functions and improved detection sensitivity. Here we report a similar technique based on a pair of RNA-DNA hybrids that can be used generally for triggering different split functionalities. Individually, each hybrid is inactive but when two c ...[more]