Ontology highlight
ABSTRACT:
SUBMITTER: Padovani D
PROVIDER: S-EPMC3620099 | biostudies-literature | 2013 Jan-Mar
REPOSITORIES: biostudies-literature
Padovani Dominique D Zeghouf Mahel M Traverso José A JA Giglione Carmela C Cherfils Jacqueline J
Small GTPases 20130101 1
Small GTP-binding proteins of the Arf family (Arf GTPases) interact with multiple cellular partners and with membranes to regulate intracellular traffic and organelle structure. Understanding the underlying molecular mechanisms requires in vitro biochemical assays to test for regulations and functions. Such assays should use proteins in their cellular form, which carry a myristoyl lipid attached in N-terminus. N-myristoylation of recombinant Arf GTPases can be achieved by co-expression in E. col ...[more]