Characterising the biology of novel lytic bacteriophages infecting multidrug resistant Klebsiella pneumoniae.

ABSTRACT:

Background

Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. The increased incidence of antimicrobial resistance in these species has propelled the need for alternate/combination therapeutic regimens to aid clinical treatment. Bacteriophage therapy forms one of these alternate strategies.

Methods

Electron microscopy, burst size, host range, sensitivity of phage particles to temperature, chloroform, pH, and restriction digestion of phage DNA were used to characterize Klebsiella phages.

Results and conclusions

Of the 32 isolated phages eight belonged to the family Myoviridae, eight to the Siphoviridae whilst the remaining 16 belonged to the Podoviridae. The host range of these phages was characterised against

SUBMITTER: Kesik-Szeloch A 

PROVIDER: S-EPMC3620542 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature