Dataset Information

Performance in omics analyses of blood samples in long-term storage: opportunities for the exploitation of existing biobanks in environmental health research.

ABSTRACT:

Background

The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown.

Objectives

We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles.

Methods

We collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for ? 24 hr before fractionating them into buffy coat, erythrocytes, and plasma and freezing the fractions at -80oC or in liquid nitrogen. We developed methodology for isolating RNA from the buffy coats and conducted omic analyses. Finally, we analyzed analogous samples from the EPIC-Italy and Northern Sweden Health and Disease Study biobanks.

Results

Microarray-quality RNA could be isolated from buffy coats (including most biobank samples) that had been frozen within 8 hr of blood collection by thawing the samples in RNA preservative. Different anticoagulants influenced the metabolomic, proteomic, and to a lesser extent transcriptomic profiles. Transcriptomic profiles were most affected by the delay (as little as 2 hr) before blood fractionation, whereas storage temperature had minimal impact. Effects on metabolomic and proteomic profiles were noted in samples processed ? 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years.

Conclusions

Most samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.

SUBMITTER: Hebels DG

PROVIDER: S-EPMC3620742 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Publications

Performance in omics analyses of blood samples in long-term storage: opportunities for the exploitation of existing biobanks in environmental health research.

Hebels Dennie G A J DG Georgiadis Panagiotis P Keun Hector C HC Athersuch Toby J TJ Vineis Paolo P Vermeulen Roel R Portengen Lützen L Bergdahl Ingvar A IA Hallmans Göran G Palli Domenico D Bendinelli Benedetta B Krogh Vittorio V Tumino Rosario R Sacerdote Carlotta C Panico Salvatore S Kleinjans Jos C S JC de Kok Theo M C M TM Smith Martyn T MT Kyrtopoulos Soterios A SA

Environmental health perspectives 20130205 4

<h4>Background</h4>The suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown.<h4>Objectives</h4>We evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles.<h4>Methods</h4>We collected fresh blood samples without R ...[more]

PMID: 23384616

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Dataset Information

Performance in omics analyses of blood samples in long-term storage: opportunities for the exploitation of existing biobanks in environmental health research.

Background

Objectives

Methods

Results

Conclusions

Publications

Performance in omics analyses of blood samples in long-term storage: opportunities for the exploitation of existing biobanks in environmental health research.

Similar Datasets

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