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Deep-tissue focal fluorescence imaging with digitally time-reversed ultrasound-encoded light.

ABSTRACT: Fluorescence imaging is one of the most important research tools in biomedical sciences. However, scattering of light severely impedes imaging of thick biological samples beyond the ballistic regime. Here we directly show focusing and high-resolution fluorescence imaging deep inside biological tissues by digitally time-reversing ultrasound-tagged light with high optical gain (~5×10(5)). We confirm the presence of a time-reversed optical focus along with a diffuse background-a corollary of partial phase conjugation-and develop an approach for dynamic background cancellation. To illustrate the potential of our method, we image complex fluorescent objects and tumour microtissues at an unprecedented depth of 2.5 mm in biological tissues at a lateral resolution of 36 ?m×52 ?m and an axial resolution of 657 ?m. Our results set the stage for a range of deep-tissue imaging applications in biomedical research and medical diagnostics.

SUBMITTER: Wang YM

PROVIDER: S-EPMC3621452 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Deep-tissue focal fluorescence imaging with digitally time-reversed ultrasound-encoded light.

Wang Ying Min YM Judkewitz Benjamin B Dimarzio Charles A CA Yang Changhuei C

Nature communications 20120626

Fluorescence imaging is one of the most important research tools in biomedical sciences. However, scattering of light severely impedes imaging of thick biological samples beyond the ballistic regime. Here we directly show focusing and high-resolution fluorescence imaging deep inside biological tissues by digitally time-reversing ultrasound-tagged light with high optical gain (~5×10(5)). We confirm the presence of a time-reversed optical focus along with a diffuse background-a corollary of partia ...[more]

PMID: 22735456

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