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Sticky nanoparticles: a platform for siRNA delivery by a bis(zinc(II) dipicolylamine)-functionalized, self-assembled nanoconjugate.

ABSTRACT: Delivering the goods: Multifunctional, self-assembled, polymeric nanoparticles for the simultaneous delivery of small-molecule drugs and siRNA have been synthesized. The nanoparticles are composed of biodegradable hyaluronic acid, for tumor targeting and cellular delivery, and a high siRNA binding affinity is provided by a Zn(II)-dipicolylamine analogue as an artificial phosphate-binding receptor (see scheme).

SUBMITTER: Liu G

PROVIDER: S-EPMC3622591 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Sticky nanoparticles: a platform for siRNA delivery by a bis(zinc(II) dipicolylamine)-functionalized, self-assembled nanoconjugate.

Liu Gang G Choi Ki Young KY Bhirde Ashwinkumar A Swierczewska Magdalena M Yin Juan J Lee Sang Wook SW Park Jae Hyung JH Hong Jong In JI Xie Jin J Niu Gang G Kiesewetter Dale O DO Lee Seulki S Chen Xiaoyuan X

Angewandte Chemie (International ed. in English) 20111123 2

Delivering the goods: Multifunctional, self-assembled, polymeric nanoparticles for the simultaneous delivery of small-molecule drugs and siRNA have been synthesized. The nanoparticles are composed of biodegradable hyaluronic acid, for tumor targeting and cellular delivery, and a high siRNA binding affinity is provided by a Zn(II)-dipicolylamine analogue as an artificial phosphate-binding receptor (see scheme). ...[more]

PMID: 22110006

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Project description:Herein, bis(zinc(II)-dipicolylamine) functionalized sub-2 μm core-shell silica microspheres (SiO2@DpaZn) were tailored for N-phosphopeptide enrichment at pH 7.7. Contributed by the coordination bonding between Zn(II) and phosphate groups with the KD value of 13.14 μM, N-phosphopeptides could be captured efficiently under neutral conditions. Moreover, contributed by the core-shell structure of SiO2@DpaZn, the fast on-tip enrichment ensured the recovery of N-phosphopeptides up to 44.1%, 3-fold higher than that obtained by in-solution capture. Moreover, 40 determined N-pho sites on 32 substrates were identified in E. coli lysates, among which five had kinase activity. All these results demonstrate that our method is beneficial to promote the discovery of N-phosphoproteins with significant biological functions.

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Bis(zinc(II)-dipicolylamine) functionalized sub-2 μm core-shell microspheres for analysis of N-phosphoproteome

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