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MiRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation.

ABSTRACT: This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKC?, GSK3? phosphorylation (GSK3?(P)) and ?-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKC? inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3?(P) and ?-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3? phosphorylation and ?-catenin production.

SUBMITTER: Vinas JL 

PROVIDER: S-EPMC3622609 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature

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miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation.

Viñas Jose Luis JL   Ventayol Marina M   Brüne Bernhard B   Jung Michaela M   Sola Anna A   Pi Felip F   Mastora Chrysoula C   Hotter Georgina G  

PloS one 20130410 4

This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin exp  ...[more]

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