Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.
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ABSTRACT:
SUBMITTER: Giebler J
PROVIDER: S-EPMC3623158 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.
Giebler Julia J Wick Lukas Y LY Schloter Michael M Harms Hauke H Chatzinotas Antonis A
Applied and environmental microbiology 20130301 9
Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent. ...[more]
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