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Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.

ABSTRACT: Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent.

SUBMITTER: Giebler J 

PROVIDER: S-EPMC3623158 | biostudies-literature | 2013 May

REPOSITORIES: biostudies-literature

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Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.

Giebler Julia J   Wick Lukas Y LY   Schloter Michael M   Harms Hauke H   Chatzinotas Antonis A  

Applied and environmental microbiology 20130301 9

Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly inconsistent. ...[more]

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