Measuring cell mechanics by optical alignment compression cytometry.
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ABSTRACT:
SUBMITTER: Roth KB
PROVIDER: S-EPMC3623556 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Publications
Measuring cell mechanics by optical alignment compression cytometry.
Roth Kevin B KB Eggleton Charles D CD Neeves Keith B KB Marr David W M DW
Lab on a chip 20130401 8
To address the need for a high throughput, non-destructive technique for measuring individual cell mechanical properties, we have developed optical alignment compression (OAC) cytometry. OAC combines hydrodynamic drag in an extensional flow microfluidic device with optical forces created with an inexpensive diode laser to induce measurable deformations between compressed cells. In this, a low-intensity linear optical trap aligns incoming cells with the flow stagnation point allowing hydrodynamic ...[more]
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