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ABSTRACT: Background
Hepcidin is a central regulator of iron metabolism. Serum hepcidin levels are increased in patients with renal insufficiency, which may contribute to anemia. Urine hepcidin was found to be increased in some patients after cardiac surgery, and these patients were less likely to develop acute kidney injury. It has been suggested that urine hepcidin may protect by attenuating heme-mediated injury, but processes involved in urine hepcidin excretion are unknown.Methods
To assess the role of tubular reabsorption we compared fractional excretion (FE) of hepcidin-25 with FE of ?2-microglobulin (?(2)m) in 30 patients with various degrees of tubular impairment due to chronic renal disease. To prove that hepcidin is reabsorbed by the tubules in a megalin-dependent manner, we measured urine hepcidin-1 in wild-type and kidney specific megalin-deficient mice. Lastly, we evaluated FE of hepcidin-25 and ?(2)m in 19 patients who underwent cardiopulmonary bypass surgery. Hepcidin was measured by a mass spectrometry assay (MS), whereas ?(2)m was measured by ELISA.Results
In patients with chronic renal disease, FE of hepcidin-25 was strongly correlated with FE of ?(2)m (r = 0.93, P <0.01). In megalin-deficient mice, urine hepcidin-1 was 7-fold increased compared to wild-type mice (p < 0.01) indicating that proximal tubular reabsorption occurs in a megalin- dependent manner. Following cardiac surgery, FE of hepcidin-25 increased despite a decline in FE of ?(2)m, potentially indicating local production at 12-24 hours.Conclusions
Hepcidin-25 is reabsorbed by the renal tubules and increased urine hepcidin-25 levels may reflect a reduction in tubular uptake. Uncoupling of FE of hepcidin-25 and ?(2)m in cardiac surgery patients suggests local production.
SUBMITTER: Peters HP
PROVIDER: S-EPMC3623618 | biostudies-literature | 2013 Mar
REPOSITORIES: biostudies-literature
Peters Hilde P E HP Laarakkers Coby M M CM Pickkers Peter P Masereeuw Rosalinde R Boerman Otto C OC Eek Annemarie A Cornelissen Elisabeth A M EA Swinkels Dorine W DW Wetzels Jack F M JF
BMC nephrology 20130325
<h4>Background</h4>Hepcidin is a central regulator of iron metabolism. Serum hepcidin levels are increased in patients with renal insufficiency, which may contribute to anemia. Urine hepcidin was found to be increased in some patients after cardiac surgery, and these patients were less likely to develop acute kidney injury. It has been suggested that urine hepcidin may protect by attenuating heme-mediated injury, but processes involved in urine hepcidin excretion are unknown.<h4>Methods</h4>To a ...[more]