Unknown

Dataset Information

0

Hsp65-producing Lactococcus lactis prevents experimental autoimmune encephalomyelitis in mice by inducing CD4+LAP+ regulatory T cells.


ABSTRACT: Heat shock proteins (Hsps) participate in the cellular response to stress and they are hiperexpressed in inflammatory conditions. They are also known to play a major role in immune modulation, controlling, for instance, autoimmune responses. In this study, we showed that oral administration of a recombinant Lactococcus lactis strain that produces and releases LPS-free Hsp65 prevented the development of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice. This was confirmed by the reduced inflammatory cell infiltrate and absence of injury signs in the spinal cord. The effect was associated with reduced IL-17 and increased IL-10 production in mesenteric lymph node and spleen cell cultures. Hsp65-producing-L. lactis-fed mice had a remarkable increase in the number of natural and inducible CD4+Foxp3+ regulatory T (Treg) cells and CD4+LAP+ (Latency-associated peptide) Tregs - which express the membrane-bound TGF-? - in spleen, inguinal and mesenteric lymph nodes as well as in spinal cord. Moreover, many Tregs co-expressed Foxp3 and LAP. In vivo depletion of LAP+ cells abrogated the effect of Hsp65-producing L. lactis in EAE prevention and worsened disease in medium-fed mice. Thus, Hsp65-L.lactis seems to boost this critical regulatory circuit involved in controlling EAE development in mice.

SUBMITTER: Rezende RM 

PROVIDER: S-EPMC3623677 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Hsp65-producing Lactococcus lactis prevents experimental autoimmune encephalomyelitis in mice by inducing CD4+LAP+ regulatory T cells.

Rezende Rafael M RM   Oliveira Rafael P RP   Medeiros Samara R SR   Gomes-Santos Ana C AC   Alves Andrea C AC   Loli Flávia G FG   Guimarães Mauro A F MA   Amaral Sylvia S SS   da Cunha André P AP   Weiner Howard L HL   Azevedo Vasco V   Miyoshi Anderson A   Faria Ana M C AM  

Journal of autoimmunity 20120828


Heat shock proteins (Hsps) participate in the cellular response to stress and they are hiperexpressed in inflammatory conditions. They are also known to play a major role in immune modulation, controlling, for instance, autoimmune responses. In this study, we showed that oral administration of a recombinant Lactococcus lactis strain that produces and releases LPS-free Hsp65 prevented the development of experimental autoimmune encephalomyelitis (EAE) in C57BL/6 mice. This was confirmed by the red  ...[more]

Similar Datasets

| S-EPMC5277002 | biostudies-literature
| S-EPMC7538670 | biostudies-literature
| S-EPMC8407701 | biostudies-literature
| S-EPMC5674443 | biostudies-literature
| S-EPMC4472905 | biostudies-literature
| S-EPMC4183873 | biostudies-literature
| S-EPMC7201567 | biostudies-literature
| S-EPMC10377672 | biostudies-literature
| S-EPMC5071574 | biostudies-literature
| S-EPMC6796575 | biostudies-literature