Unknown

Dataset Information

0

Fly cryptochrome and the visual system.


ABSTRACT: Cryptochromes are flavoproteins, structurally and evolutionarily related to photolyases, that are involved in the development, magnetoreception, and temporal organization of a variety of organisms. Drosophila CRYPTOCHROME (dCRY) is involved in light synchronization of the master circadian clock, and its C terminus plays an important role in modulating light sensitivity and activity of the protein. The activation of dCRY by light requires a conformational change, but it has been suggested that activation could be mediated also by specific "regulators" that bind the C terminus of the protein. This C-terminal region harbors several protein-protein interaction motifs, likely relevant for signal transduction regulation. Here, we show that some functional linear motifs are evolutionarily conserved in the C terminus of cryptochromes and that class III PDZ-binding sites are selectively maintained in animals. A coimmunoprecipitation assay followed by mass spectrometry analysis revealed that dCRY interacts with Retinal Degeneration A (RDGA) and with Neither Inactivation Nor Afterpotential C (NINAC) proteins. Both proteins belong to a multiprotein complex (the Signalplex) that includes visual-signaling molecules. Using bioinformatic and molecular approaches, dCRY was found to interact with Neither Inactivation Nor Afterpotential C through Inactivation No Afterpotential D (INAD) in a light-dependent manner and that the CRY-Inactivation No Afterpotential D interaction is mediated by specific domains of the two proteins and involves the CRY C terminus. Moreover, an impairment of the visual behavior was observed in fly mutants for dCRY, indicative of a role, direct or indirect, for this photoreceptor in fly vision.

SUBMITTER: Mazzotta G 

PROVIDER: S-EPMC3625353 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications


Cryptochromes are flavoproteins, structurally and evolutionarily related to photolyases, that are involved in the development, magnetoreception, and temporal organization of a variety of organisms. Drosophila CRYPTOCHROME (dCRY) is involved in light synchronization of the master circadian clock, and its C terminus plays an important role in modulating light sensitivity and activity of the protein. The activation of dCRY by light requires a conformational change, but it has been suggested that ac  ...[more]

Similar Datasets

| S-EPMC6135900 | biostudies-other
2022-01-01 | GSE167266 | GEO
| S-EPMC10882054 | biostudies-literature
| S-EPMC9074256 | biostudies-literature
| S-EPMC5448152 | biostudies-literature
| S-EPMC5730180 | biostudies-literature
| S-EPMC8136689 | biostudies-literature
2020-12-17 | GSE163311 | GEO
| S-EPMC5893613 | biostudies-literature
| S-EPMC4460454 | biostudies-literature