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DNA-mediated assembly of protein heterodimers on membrane surfaces.

ABSTRACT: We present a method based on self-assembling oligonucleotides to anchor proteins to a supported membrane surface. This anchoring method allows control of the surface density of multiple proteins. By incorporating additional recognition sequences into the DNA linkers, defined heterodimers can be produced upon the addition of a heterospecific DNA cross-linking strand. Characterization by fluorescence cross-correlation spectroscopy (FCCS) confirmed lateral mobility and the formation of specific heterodimers. We further demonstrate that proteins linked in this manner as either monomers or dimers can form functional interfaces with living cells.

SUBMITTER: Coyle MP

PROVIDER: S-EPMC3626235 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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DNA-mediated assembly of protein heterodimers on membrane surfaces.

Coyle Michael P MP Xu Qian Q Chiang Samantha S Francis Matthew B MB Groves Jay T JT

Journal of the American Chemical Society 20130326 13

We present a method based on self-assembling oligonucleotides to anchor proteins to a supported membrane surface. This anchoring method allows control of the surface density of multiple proteins. By incorporating additional recognition sequences into the DNA linkers, defined heterodimers can be produced upon the addition of a heterospecific DNA cross-linking strand. Characterization by fluorescence cross-correlation spectroscopy (FCCS) confirmed lateral mobility and the formation of specific het ...[more]

PMID: 23530555

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