Ontology highlight
ABSTRACT:
SUBMITTER: Meckler JF
PROVIDER: S-EPMC3627578 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Meckler Joshua F JF Bhakta Mital S MS Kim Moon-Soo MS Ovadia Robert R Habrian Chris H CH Zykovich Artem A Yu Abigail A Lockwood Sarah H SH Morbitzer Robert R Elsäesser Janett J Lahaye Thomas T Segal David J DJ Baldwin Enoch P EP
Nucleic acids research 20130213 7
Transcription activator-like effectors (TALEs) have revolutionized the field of genome engineering. We present here a systematic assessment of TALE DNA recognition, using quantitative electrophoretic mobility shift assays and reporter gene activation assays. Within TALE proteins, tandem 34-amino acid repeats recognize one base pair each and direct sequence-specific DNA binding through repeat variable di-residues (RVDs). We found that RVD choice can affect affinity by four orders of magnitude, wi ...[more]