Ontology highlight
ABSTRACT:
SUBMITTER: Ching RW
PROVIDER: S-EPMC3628506 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Ching Reagan W RW Ahmed Kashif K Boutros Paul C PC Penn Linda Z LZ Bazett-Jones David P DP
The Journal of cell biology 20130401 2
Important insights into nuclear function would arise if gene loci physically interacting with particular subnuclear domains could be readily identified. Immunofluorescence microscopy combined with fluorescence in situ hybridization (immuno-FISH), the method that would typically be used in such a study, is limited by spatial resolution and requires prior assumptions for selecting genes to probe. Our new technique, immuno-TRAP, overcomes these limitations. Using promyelocytic leukemia nuclear bodi ...[more]