IL-13-producing BLT1-positive CD8 cells are increased in asthma and are associated with airway obstruction.

ABSTRACT:

Background

The role of CD8 T lymphocytes in the pathogenesis of asthma is not well understood. We investigated whether a subset of IL-13-producing BLT1-positive CD8 T lymphocytes are present in asthmatic airways and are associated with impaired lung function.

Methods

Bronchoalveolar lavage (BAL) cells were obtained from asthmatic (n = 39) and healthy control (n = 28) subjects. Cells were stimulated with phorbol ester and ionomycin in the presence of brefeldin A and stained for CD8, BLT1, and intracellular IL-13. The frequency of IL-13-producing BLT1-positive CD8 T lymphocytes was compared between the two groups and related to lung function, serum IgE levels, and reticular basement membrane (RBM) thickness.

Results

A subset of CD8 T lymphocytes expressing BLT1 and prod

SUBMITTER: Dakhama A 

PROVIDER: S-EPMC3630251 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature