PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line.
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ABSTRACT:
SUBMITTER: Wang BS
PROVIDER: S-EPMC3631091 | biostudies-literature | 2013 May
REPOSITORIES: biostudies-literature
Publications
PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line.
Wang Bo-Shi BS Yang Yang Y Yang Hai H Liu Yi-Zhen YZ Hao Jia-Jie JJ Zhang Yu Y Shi Zhi-Zhou ZZ Jia Xue-Mei XM Zhan Qi-Min QM Wang Ming-Rong MR
Cell stress & chaperones 20121209 3
Using a glutathione S-transferase pull-down liquid chromatography-coupled tandem mass spectrometry approach and immunoprecipitation/immunoblot analysis, we found that heat shock cognate protein 70 (Hsc70) was involved in the complex formed by atypical protein kinase Cι (PKCι) and LC3 in the esophageal cancer cell line KYSE30. Further study indicated that Hsc70 was targeted by autophagic degradation, and knockdown of PKCι down-regulated Hsc70 by promoting autophagy. PKCι knockdown sensitized cell ...[more]
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