Ontology highlight
ABSTRACT:
SUBMITTER: Yang F
PROVIDER: S-EPMC3632134 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Yang Fei F Wang Xiu-Ye XY Zhang Zhi-Min ZM Pu Jia J Fan Yu-Jie YJ Zhou Jiahai J Query Charles C CC Xu Yong-Zhen YZ
Nucleic acids research 20130305 8
Fidelity and efficiency of pre-mRNA splicing are critical for generating functional mRNAs, but how such accuracy in 5' splice site (SS) selection is attained is not fully clear. Through a series of yeast genetic screens, we isolated alleles of prp28 that improve splicing of suboptimal 5'SS substrates, demonstrating that WT-Prp28p proofreads, and consequently rejects, poor 5'SS. Prp28p is thought to facilitate the disruption of 5'SS-U1 snRNA pairing to allow for 5'SS-U6 snRNA pairing in the catal ...[more]